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Effect of inducer addition on production of 4,5-dihydroxyphthalate by Pseudomonas testosteroni

✍ Scribed by Akihiko Sakurai; Mikio Sakakibara

Book ID: 101265272
Publisher: Wiley (John Wiley & Sons)
Year: 2000
Tongue: English
Weight: 63 KB
Volume: 75
Category: Article
ISSN: 0268-2575
DOI: 10.1002/(sici)1097-4660(200001)75:1<107::aid-jctb180>3.0.co;2-q

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✦ Synopsis

The effect of the addition method of an inducer (o-phthalate) for the enhancement of 4,5dihydroxyphthalate (DHP) production was examined using Pseudomonas testosteroni. The reduction of the lag phase seen when inducer was added in the ®rst 10 h of growth culture did not affect the maximum production rate of DHP. The optimal inducer concentration was about 2 g dm À3 and the rate of DHP production was about four times higher that achieved in the absence of inducer. The fed-batch production of DHP was also carried out and 8 g dm À3 of DHP was obtained.

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