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Process for the preparation of l-serine by an enzymatic method

โœ Scribed by Fukuhara


Publisher
Elsevier Science
Year
1996
Tongue
English
Weight
122 KB
Volume
18
Category
Article
ISSN
0141-0229

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## B i o l o g i c a l l y l a b e l l e d [l-14C) g l y c e r o l ( L -[ l -' " C ) g l y c e r o l ) 048 prepared by a two s t e p p r o c e s s . S t e p one i n v o 1 v e d t h e p r e p a r a t i o n of [I -C ] g 2 y ce r o 1 3 -p ho sp ha t e from l4C02 and D-ribuloee 1,5 -diphoephate u s i

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Methods are described for the use of L-serine dehydratase purified from Clostridium acidiurici for the determination of L-serine concentration and L[ 14C]serine specific radioactivity in sheep plasma. A spectrophotometric assay using this enzyme accurately measured the concentration of L-serine in s

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An easy, rapid, and accurate enzymatic assay method for L-serine was established involving two enzymes, serine-glyoxylate aminotransferase (SGAT, EC 2.6.1.45) and hydroxypyruvate reductase (HPR, EC 1.1.1.81), in the serine pathway of the methylotrophic bacterium, Hyphomicrobium methylovorum (IFO 141

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โœ R. Emaus; L.L. Bieber ๐Ÿ“‚ Article ๐Ÿ“… 1982 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 417 KB

A rapid method for the preparation of [ I-'4C]acetyl-~-carnitine is described. The method involves exchange of [ I-'%]acetic acid into a pool of unlabeled acetyl-L-carnitine using the enzymes acetyl-CoA synthetase and carnitine acetyltransferase. After isotopic equilibrium is attained, radioactive a