Problems in the use of [Me-3H]thymidine for the measurement of DNA synthesis
β Scribed by David F. Goldspink; Alfred L. Goldberg
- Book ID
- 115745306
- Publisher
- Elsevier Science
- Year
- 1973
- Weight
- 663 KB
- Volume
- 299
- Category
- Article
- ISSN
- 0005-2787
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## Abstract An enzymatic method for synthesis of tritiumβlabelled thymidineβ5β²βtriphosphate is described. The procedure utilizes a nucleoside phosphotransferase from wheat germ and a partially purified thymidylate kinase from calf thymus. The product is isolated by ionβexchange chromatography on DE