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Measurement of bacterial growth rates on the rhizoplane using3H-thymidine incorporation into DNA

✍ Scribed by E. Bååth; T. Johansson

Publisher
Springer
Year
1990
Tongue
English
Weight
529 KB
Volume
126
Category
Article
ISSN
0032-079X

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✦ Synopsis

Bacterial growth rates on the rhizoplane of rape seedlings grown in sand were determined using 3H-thymidine incorporation into DNA. Axenic roots incorporated thymidine into DNA, which had to be subtracted from values for roots with associated bacteria. Thymidine incorporation into rhizoplane bacterial DNA ranged between 0.6 and 1.4 pmol thymidine h -1 root-t for 6 to 26-day-old plants. Using a conversion factor, the turnover time of bacteria was calculated to decrease from 9.2 h for 6-day-old plants to 160 h for 26-day-old plants. A similar value was found for rhizosphere bacteria of plants grown for 26 days in natural soil.

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✍ John E. Sherwin; Solon A. Gordon 📂 Article 📅 1974 🏛 Springer-Verlag 🌐 English ⚖ 454 KB

Preincubation of apical segments of etiolated peas (Pisum sativum L.) in indole-3-acetic acid (IAA) results in an inhibition of the incorporation of [aH] thymidine ([~H]TdR) into DNA. Preincubation in IAA for 4 h led ~o an inhibition of [3H]TdR incorporation only at the highest concentration of IAA