𝔖 Bobbio Scriptorium
✦   LIBER   ✦

PROBLEMS CONCERNING “NONCULTIVABLE” MYCOPLASMA CONTAMINANTS IN TISSUE CULTURES

✍ Scribed by Hope E. Hopps; Barbara C. Meyer; Michael F. Barile; R. A. DelGiudice


Book ID
114880583
Publisher
John Wiley and Sons
Year
1973
Tongue
English
Weight
989 KB
Volume
225
Category
Article
ISSN
0890-6564

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The in situ staining method of Chen (1977) for the detection of mycoplasma contaminants in tissue cultures was tested in cultures of human skin fibroblasts after controlled contamination with Mycoplasma arginini. It is concluded that this method is reliable only at infection rates of 100% or higher,