Primary culture of proximal tubule cells in defined medium
β Scribed by Mary L. Taub
- Publisher
- Springer
- Year
- 1985
- Tongue
- English
- Weight
- 453 KB
- Volume
- 9
- Category
- Article
- ISSN
- 1573-0603
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π SIMILAR VOLUMES
The properties of primary rabbit kidney proximal tubule cells in glucose-free serum-free medium have been examined. Primary rabbit kidney proximal tubule cells were observed to grow at the same rate, 1 .O doublingsiday, both in glucosefree and in glucose-supplemented medium. Growth in glucose-free m
## Abstract Primary cultures of baby mouse kidney epithelial cells can grow without fibroblast overgrowth in a hormoneβsupplemented serumβfree medium (Medium Kβ1) designed for an established kidney epithelial cell line, MDCK. The five supplements in Medium Kβ1 are insulin, transferrin, PGE~1~, T~3~
The effects of estrogens on the growth and function of primary rabbit kidney proximal tubule (RPT) cells have been examined in hormonally defined phenol red-free medium. 17β€-estradiol was observed to stimulate growth at dosages as low as 10 -10 M. The growth stimulatory effects of 17β€-estradiol were