Presence of circulating anti-c-myb oncogene product antibodies in human sera

✍ Scribed by Irène Sorokine; Kamel Ben-Mahrez; Annie Bracone; Dominique Thierry; Shunsuke Ishii; Fumio Imamoto; Masamichi Kohiyama

• Publisher: John Wiley and Sons
• Year: 1991
• Tongue: French
• Weight: 575 KB
• Volume: 47
• Category: Article
• ISSN: 0020-7136
• DOI: 10.1002/ijc.2910470506

✦ Synopsis

Bacterially expressed mouse c-myb protein was purified from E. coliextracts and used as antigen to screen human sera for circulating anti-c-my6 oncogene product antibodies. Using Western blotting, we have found that human sera contain IgG antibodies to the c-my6 gene product. The percentage of positive sera in cancer patients appears to be dependent upon cancer type and is significantly higher in breast-cancer patients than in normal donors: 43% of sera from breast-cancer patients are positive, whereas in neuroblastoma cancer patients the production of IgG anti-c-myb appears to be rare. No significant correlation was observed between the presence of circulating anti-c-myb antibodies and the expression of the c-myb gene in breast tumors.