Preparation, titration, and storage of chloroacetaldehyde for fluorometric determination of adenine and its derivatives
β Scribed by McCann, William P.; Hall, Leo M.; Nonidez, William K.
- Book ID
- 121322047
- Publisher
- American Chemical Society
- Year
- 1983
- Tongue
- English
- Weight
- 286 KB
- Volume
- 55
- Category
- Article
- ISSN
- 0003-2700
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## Abstract Chloroacetaldehyde, which reacts selectively with cytosine and adenine nucleotides to give fluorescent ethenoβbridged derivatives, was synthesized bearing a ^14^C label. The chloroacetaldehyde lβ^14^C, prepared from glycerolβ2β^14^C, was used to form ethenoβbridged adenine and cytosine
Modified procedures for separating and determining adenine nucleotides are described. Separation was achieved by one-dimensional thin-layer chromatography on polyethyleneimine-cellulose plates in 1.4 M LiCl after prewashing the spotted plate in deionized water. The fluorometric procedure for determi