Preparation of α-aminonitrones from 3-imidazoline-3-oxide α-methoxynitrones
✍ Scribed by G. I. Shchukin; I. A. Grigor'ev; L. A. Vishnivetskaya; L. B. Volodarskii
- Book ID
- 112448756
- Publisher
- Springer
- Year
- 1988
- Tongue
- English
- Weight
- 61 KB
- Volume
- 37
- Category
- Article
- ISSN
- 1573-9171
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A method is described for preparing highly purified 3a-and 3P-hydroxysteroid dehydrogenases (EC 1.1.1.50 and EC 1.1.1.145, respectively), essentially uncontaminated with one another, from extracts of a steroid-induced Pseudomonas species. These enzymes are suitable for the microanalysis of 3a-hydrox