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Preparation of highly purified 3α- and 3β-hydroxysteroid dehydrogenases from Pseudomonas sp

✍ Scribed by Mikio Shikita; Paul Talalay

Publisher
Elsevier Science
Year
1979
Tongue
English
Weight
544 KB
Volume
95
Category
Article
ISSN
0003-2697

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✦ Synopsis

A method is described for preparing highly purified 3a-and 3P-hydroxysteroid dehydrogenases (EC 1.1.1.50 and EC 1.1.1.145, respectively), essentially uncontaminated with one another, from extracts of a steroid-induced Pseudomonas species. These enzymes are suitable for the microanalysis of 3a-hydroxy-, 3@hydroxy-, and 3-ketosteroids.

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