𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Preparation of Weak Cation Exchange Monolithic Capillary Column and Its Application for Protein Separation

✍ Scribed by Ming-Yu DING; Rui ZHENG; Hong PENG

Book ID
104452559
Publisher
Elsevier Science
Year
2009
Tongue
Chinese
Weight
299 KB
Volume
37
Category
Article
ISSN
1872-2040

No coin nor oath required. For personal study only.

✦ Synopsis

A polymer-based capillary monolithic support was prepared by in situ copolymerization using glycidyl methacrylate (GMA) as monomer, ethylene dimethacrylate (EDMA) as crosslinking reagent, and a mixture of 1-propanol, 1,4-butanediol, and water as porogen solvent. The optimal ratio of above reactants in volume is GMA : EDMA : 1-propanol : 1,4-butanediol : water = 0.32:0.08:0.35:0.2:0.05. A macroporous monolithic support with 100-300 nm pore size was obtained under the reacting conditions of 24 h and 60 ºC. The monolithic support was modified with iminodiacetic acid (IDA) and a weak cation-exchange capillary monolithic column was obtained. The optimal modifying conditions were: reaction time of 24 h, reaction temperature of 75 C, and reaction pH value of 11.0. The three model proteins, albumin egg, trypsin, and lysozyme, could be separated on this weak cation-exchange capillary monolithic column with a length of only 50 mm within 13 min.

📜 SIMILAR VOLUMES

Preparation of a monolithic column for w
Preparation of a monolithic column for weak cation exchange chromatography and its application in the separation of biopolymers
✍ Yinmao Wei; Xiaodong Huang; Ruixian Liu; Yehua Shen; Xindu Geng 📂 Article 📅 2006 🏛 John Wiley and Sons 🌐 English ⚖ 516 KB

## Abstract A procedure for the preparation of a monolithic column for weak cation exchange chromatography was presented. The structure of the monolithic column was evaluated by mercury intrusion. The hydrodynamic and chromatographic properties of the monolithic column – such as back pressures at d

Weak cation-exchange monolithic column f
Weak cation-exchange monolithic column for capillary liquid chromatography of peptides and proteins
✍ Xin Chen; H. Dennis Tolley; Milton L. Lee 📂 Article 📅 2011 🏛 John Wiley and Sons 🌐 English ⚖ 432 KB

## Abstract A stable poly(2‐carboxyethyl acrylate‐__co__‐poly(ethylene glycol) diacrylate) monolith was synthesized inside a 75‐μm id capillary by direct __in situ__ photo‐initiated polymerization in a binary porogenic solvent consisting of methanol and ethyl ether. The resulting monolith was evalu

Preparation of monolithic silica column
Preparation of monolithic silica column with strong cation-exchange stationary phase for capillary electrochromatography
✍ Chuanhui Xie; Jiwei Hu; Hua Xiao; Xingye Su; Jing Dong; Ruijun Tian; Zhike He; H 📂 Article 📅 2005 🏛 John Wiley and Sons 🌐 English ⚖ 415 KB

## Abstract A monolithic silica based strong cation‐exchange stationary phase was successfully prepared for capillary electrochromatography. The monolithic silica matrix from a sol‐gel process was chemically modified by treatment with 3‐mercaptopropyltrimethoxysilane followed by a chemical oxidatio

Polymeric strong cation-exchange monolit
Polymeric strong cation-exchange monolithic column for capillary liquid chromatography of peptides and proteins
✍ Xin Chen; H. Dennis Tolley; Milton L. Lee 📂 Article 📅 2009 🏛 John Wiley and Sons 🌐 English ⚖ 727 KB

## Abstract A strong cation‐exchange (SCX) monolithic stationary phase was prepared in 75 μm id capillaries by direct __in situ__ polymerization of sulfopropyl methacrylate and polyethylene glycol diacrylate in a ternary porogen system consisting of methanol, cyclohexanol, and water. The resulting

Preparation and evaluation of rigid poro
Preparation and evaluation of rigid porous polyacrylamide-based strong cation-exchange monolithic columns for capillary electrochromatography
✍ Jing Dong; Junjie Ou; Xiaoli Dong; Renan Wu; Mingliang Ye; Hanfa Zou 📂 Article 📅 2007 🏛 John Wiley and Sons 🌐 English ⚖ 1000 KB

## Abstract A CEC monolithic column with strong cation‐exchange (SCX) stationary phase based on hydrophilic monomers was prepared by __in situ__ polymerization of acrylamide, methylenebisacrylamide, and 2‐acrylamido‐2‐methyl‐1‐propanesulfonic acid (AMPS) in a complete organic binary porogenic solve