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Polymeric strong cation-exchange monolithic column for capillary liquid chromatography of peptides and proteins

✍ Scribed by Xin Chen; H. Dennis Tolley; Milton L. Lee

Publisher
John Wiley and Sons
Year
2009
Tongue
English
Weight
727 KB
Volume
32
Category
Article
ISSN
1615-9306

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✦ Synopsis

Abstract

A strong cation‐exchange (SCX) monolithic stationary phase was prepared in 75 μm id capillaries by direct in situ polymerization of sulfopropyl methacrylate and polyethylene glycol diacrylate in a ternary porogen system consisting of methanol, cyclohexanol, and water. The resulting monolith exhibited good dynamic binding capacity, fast kinetic adsorption of proteins, and high permeability. The monolith had a dynamic binding capacity of ˜52 mg/mL of column volume for lysozyme and cytochrome C. The monolith was evaluated for SCX capillary LC of synthetic peptides, natural peptides, and protein standards. Fast separation of proteins was achieved in less than 4 min. The average peak capacity for peptides was 28 using a relatively steep gradient when hydrophobic interactions were suppressed with 40% acetonitrile.

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