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Preparation and evaluation of rigid porous polyacrylamide-based strong cation-exchange monolithic columns for capillary electrochromatography

✍ Scribed by Jing Dong; Junjie Ou; Xiaoli Dong; Renan Wu; Mingliang Ye; Hanfa Zou

Publisher
John Wiley and Sons
Year
2007
Tongue
English
Weight
1000 KB
Volume
30
Category
Article
ISSN
1615-9306

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✦ Synopsis

Abstract

A CEC monolithic column with strong cation‐exchange (SCX) stationary phase based on hydrophilic monomers was prepared by in situ polymerization of acrylamide, methylenebisacrylamide, and 2‐acrylamido‐2‐methyl‐1‐propanesulfonic acid (AMPS) in a complete organic binary porogenic solvent consisting of DMSO and dodecanol. The sulfonic groups provided by the monomer AMPS on the surface of the stationary phase generate an EOF from anode to cathode, and serve as an SCX stationary phase at the same time. The monolithic stationary phase exhibited normal‐phase chromatographic behavior for neutral analytes. For charged analytes, electrostatic interaction/repulsion with the monolith was observed. The strong SCX monolithic column has been successfully employed in the electrochromatographic separation of basic drugs, peptides, and alkaloids extracted from natural products.

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