Aiming at the direct preparation of peptide thioesters by an Fmoc solid-phase method, we searched a new deblocking reagent, which efficiently removed Fmoc groups while keeping the thioester intact. The deblocking reagent, which contains 1-methylpyrrolidine, hexamethyleneimine and HOBt in a one to on
Preparation of phosphopeptide thioesters by Fmoc-and Fmoc(2-F)-solid phase synthesis
โ Scribed by Koki Hasegawa; Yin Lin Sha; Jeong Kyu Bang; Toru Kawakami; Kenichi Akaji; Saburo Aimoto
- Publisher
- Springer Netherlands
- Year
- 2001
- Tongue
- English
- Weight
- 505 KB
- Volume
- 8
- Category
- Article
- ISSN
- 1573-3149
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๐ SIMILAR VOLUMES
Established methodology for the preparation of peptide thioesters requires the use of t-butoxycarbonyl chemistry owing to the lability of thioester linkers to the nucleophilic reagents used in Fmoc solid phase peptide synthesis. Both the greater ease of use and the broad applicability of the method
To synthesize peptide thioesters directly on a solid support for use as substrate analogues for thioesterases in non-ribosomal peptide synthases, we modified a reagent compatible with Fmoc solid-phase peptide synthesis that efficiently removes the protecting group while keeping the thioester intact.