Preparation of neural tissues and sensory structures for electron microscopy

✍ Scribed by Song, Jindan

• Publisher: Wiley (John Wiley & Sons)
• Year: 1987
• Tongue: English
• Weight: 908 KB
• Volume: 7
• Category: Article
• ISSN: 0741-0581
• DOI: 10.1002/jemt.1060070204

✦ Synopsis

This paper describes the preparation of specimens of neural tissues and sensory structures for electron microscopy. A solution of 2% paraformaldehyde and 2.5% glutaraldehyde was used as fixative. The fixative entered the spinal cord of the rhesus monkey through the aorta abdominalis and emptied into the internal ear of the guinea pig via the arteria vertebralis by the vascular perfusion method. After perfusion, the spinal cord and internal ear were removed from the columna vertebralis and temporal bone, respectively. The tissues were postfixed in 1% 0 ~0 4 .

If a desired direction of the section was required, directional embedding was applied. The application of vascular perfusion fixation, excising tissues on orientation, and embedding specimens in a certain direction makes it possible to obtain the desired tissue section and intact cellular ultrastucture.