✦ LIBER ✦

A method for preparation of immobilized cells and tissues for light and electron microscopy studies

✍ Scribed by V. Ogneva; A. Neronov

Publisher: John Wiley and Sons
Year: 1995
Tongue: English
Weight: 294 KB
Volume: 30
Category: Article
ISSN: 1059-910X
DOI: 10.1002/jemt.1070300308

No coin nor oath required. For personal study only.

✦ Synopsis

INTRODUCTION. TEM studies of isolated cell cultures are performed after processing of the samples through a number of stages.

In oun laboratory, the fixed cells are initially packed in agar, collagen and other media rendering ensuing processing of the materials easier for embedding in epoxy resin. Using this method, a greater number of cells is initially required because of attrition in the intermediate stages.

Immobil ization of cells is applied in experiments with hepatocytes, Langerhans islets (1,3), hybridomas ( 2 ) , and other cell types.

The usage of such preparations contributes to elucidation of problems in growth regulation and cell differentiation.

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