The conditions for preparing partially IV-acetylated chitosans (PNACs) by reaction of chitosan with acetic anhydride in homogeneous solution were investigated. The following conditions are recommended: chitosan, 1%; acetic acid, 0.4%; methanol, 50%; at room temperature. About 80% of the acetic anhyd
Preparation of N-acetylchitooligosaccharides by hydrolysis of chitosan with chitinase followed by N-acetylation
β Scribed by Sei-ichi Aiba
- Publisher
- Elsevier Science
- Year
- 1994
- Tongue
- English
- Weight
- 433 KB
- Volume
- 265
- Category
- Article
- ISSN
- 0008-6215
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
Note /V-Acyl derivatives of chitosan and their hydrolysis by chitinase SHlOEHlKO HIKANO AND '~.~DAHIKO M~1'StJMlJK.4
A semipreparative scale chromatography of \(\mathrm{N}\)-acetylchitooligosaccharides (GlcNAc) 2.7 on a reversedphase C-16 HPLC column is reported. The initial material was obtained by enzymatic hydrolysis of chitosan with a complex of chitinases from Streptomyces kurssanovii, followed by a complete
A simple and rapid method for the quantitative determination of N-acetyl groups in chitin-chitosan samples consists of an acid hydrolysis conducted at high temperature with a mixture of sulfuric and oxalic acids. The acetic acid formed is determined by spectrophotometry at 210 nm after separation fr
The composition and sequence of 2-acetamido-2-deoxy-beta-D-glucose (GlcNAc) and 2-amino-2-deoxy-beta-D-glucose (GlcN) residues in partially N-deacetylated chitosans, prepared under homogeneous and heterogeneous conditions, have been determined by 1H-n.m.r. spectroscopy. It was necessary to depolymer