Mass-Spectrometric Analysis of N-Acetylchitooligosaccharides Prepared through Enzymatic Hydrolysis of Chitosan

✍ Scribed by S.A. Lopatin; M.M. Ilyin; V.N. Pustobaev; Z.A. Bezchetnikova; V.P. Varlamov; V.A. Davankov

• Publisher: Elsevier Science
• Year: 1995
• Tongue: English
• Weight: 247 KB
• Volume: 227
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1006/abio.1995.1282

✦ Synopsis

A semipreparative scale chromatography of (\mathrm{N})-acetylchitooligosaccharides (GlcNAc) 2.7 on a reversedphase C-16 HPLC column is reported. The initial material was obtained by enzymatic hydrolysis of chitosan with a complex of chitinases from Streptomyces kurssanovii, followed by a complete acetylation of amino groups. Isolated (\boldsymbol{N})-acetylchitooligosaccharides were characterized by the mass-spectrometric method. On interacting with the sample, the fragments of ({ }^{252} \mathbf{C f}) caused desorption of quasi-molecular ions of the substance. All mass spectra of chitin oligomers contained intense quasi-molecular ions ([\mathbf{M}+\mathbf{N a}]^{+})and a less intense ([\mathbf{M}+\mathbf{K}]^{+})ion. Fragment ions, compared to the quasi-molecular ion ([\mathbf{M}+\mathbf{K}]^{+}), had a lower intensity and confirmed the structure of samples under study. Due to this phenomenon, an accurate analysis of both individual compounds and mixtures of (\mathrm{N})-acetylchitooligosaccharides ((n=2-7)) may be performed. 1995 Academic Press. Inc.