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A rapid method for the determination of the degree of N-acetylation of chitin-chitosan samples by acid hydrolysis and HPLC

✍ Scribed by Frédéric Niola; Nùria Basora; Esteban Chornet; Pierre François Vidal

Publisher
Elsevier Science
Year
1993
Tongue
English
Weight
599 KB
Volume
238
Category
Article
ISSN
0008-6215

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✦ Synopsis

A simple and rapid method for the quantitative determination of N-acetyl groups in chitin-chitosan samples consists of an acid hydrolysis conducted at high temperature with a mixture of sulfuric and oxalic acids. The acetic acid formed is determined by spectrophotometry at 210 nm after separation from the reaction mixture by HPLC. The method is valid for the entire range of degrees of acetylation and the results compare fairly to those of other techniques.

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