✦ LIBER ✦

Preparation of monoclonal antibodies to JC virus and their use in the diagnosis of progressive multifocal leucoeneephalopathy

✍ Scribed by Dr. W. A. Knowles; I. R. Sharp; L. Efstratiou; J. F. Hand; S. D. Gardner

Publisher: John Wiley and Sons
Year: 1991
Tongue: English
Weight: 599 KB
Volume: 34
Category: Article
ISSN: 0146-6615
DOI: 10.1002/jmv.1890340211

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Seven monoclonal antibodies (MAbs) to polyomavirus JC were produced, and one was selected for use in immunofluorescence (IF) tests on brain material from patients with suspected progressive multifocal leucoencephalopathy (PML). The selected MAb (5. 12.2) reacted by IF with JC‐infected primary human foetal glial (PHFG) cell cultures (titre 1/200,000) but not with BK‐infected human embryo lung (HEL) fibroblasts (titre <1/20). Its haemagglutination‐inhibition (HI) titre was high (>1/5 × 10^6^) against JC virus but low (<1/5)against BK virus. A diagnosis of PML was confirmed on brain biopsy or at postmortem in four patients, two of whom were also infected with human immunodeficiency virus (HIV). In one of the patients, specific detection of JC virus antigen had not been possible using our routine high titred JC and BK human sera due to interference by JC antibody present in the cerebrospinal fluid (CSF). Viral antigen was, however, detected with the MAb 5. 12.2.

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