## Abstract The modified method for biosynthesis of Lโmethionineโ^35^S and Lโcystineโ^35^S from baker's yeast (__Saccharomyces cerevisiae__) is described. The yeast was cultivated in a sulphurโdeplated medium containing carrierโfree Na~2~^35^SO~4~, under air bubbling during 24 hours. Yeast proteins
Preparation of L-Methionine-S 35 and L-Cystine-S 35 from Radioactive Yeast 1
โ Scribed by Wood, John L.; Mills, Gordon C.
- Book ID
- 126435303
- Publisher
- American Chemical Society
- Year
- 1952
- Tongue
- English
- Weight
- 93 KB
- Volume
- 74
- Category
- Article
- ISSN
- 0002-7863
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๐ SIMILAR VOLUMES
The method for the synthesis of L-cysteine hydrochloride labelled with 35S is described. L -C ~s t i n e -~~S obtained from baker's yeast was reduced with tin in hydrochloric acid and radiochemically pure L-cysteine-35S hydrochloride was isolated by ion-exchange chromatography on a column. The obta
## Abstract High specific activity Lโ^35^ Sโcysteine and Lโ^35^ Sโmethionine were synthesised by using a wild type diploid strain of baker's __yeastโSaccharomyces cerevisiae__. Yeast cells were grown in a sulphur depleted synthetic medium in which Na~2~ ^35^ SO~4~ (50 mCi/ml) was supplemented as th