Preparation of immobilized β-galactosidase by poly(vinylpyrrolidone) and the continuous hydrolysis of lactose in acid whey
✍ Scribed by Hidekatsu Maeda
- Publisher
- John Wiley and Sons
- Year
- 1975
- Tongue
- English
- Weight
- 747 KB
- Volume
- 17
- Category
- Article
- ISSN
- 0006-3592
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A novel chemical reactor, consisting of P-galactosidase from Bacillus circulans immobilized onto a ribbed membrane made from polyvinylchloride and silica, was used to hydrolyze the lactose constituent of skim milk. Multiresponse nonlinear regression methods were employed to determine the kinetic par
## Abstract Partially purified lactoses (β‐D‐galactoside galactohydrolase, EC 3.2.1.23) from __Aspergillus niger__, __Ladobacillus helveticus__, and __Saccharomyces lactis__ were immobilized on diazotized porous glass particles (mean pore diameter, 86.5 nm: particle size diameters, 75–125 μm). In a