Hydrolysis of lactose in acid whey using β-galactosidase immobilized on porous glass particles: Preparation and characterization of a reusable catalyst for the production of low-lactose dairy products
✍ Scribed by L. E. Wierzbicki; V. H. Edwards; F. V. Kosikowski
- Publisher
- John Wiley and Sons
- Year
- 1974
- Tongue
- English
- Weight
- 678 KB
- Volume
- 16
- Category
- Article
- ISSN
- 0006-3592
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✦ Synopsis
Abstract
Partially purified lactoses (β‐D‐galactoside galactohydrolase, EC 3.2.1.23) from Aspergillus niger, Ladobacillus helveticus, and Saccharomyces lactis were immobilized on diazotized porous glass particles (mean pore diameter, 86.5 nm: particle size diameters, 75–125 μm). In acid whey containing 4–4.5% lactose, A. niger lactase gave the highest activity (89 μmoles lactose hydrolyzed/g glass, min) at 55°C and pH 4.5. Glass‐immobilized A. niger laclases (lactase‐BG) retained much hydrolytic activity after storage and periodic use for 165 days at 55°C. For values of X greater than 30%, hydrolysis of 0.12__M__ lactose in acid whey by a continuous flow column packed with 2 ml of lactase‐BG particles could be correlated by X = 17.2(V/F) + 12.5 where X = lactose hydrolysis, percent of lactose originally present; V = volume of packed bed of lactase‐BG, ml; F = flow rate of acid whey, ml/min.