𝔖 Bobbio Scriptorium
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Preparation of 3H-labeled ochratoxins

✍ Scribed by Fred C. Chang; F. S. Chu

Publisher
John Wiley and Sons
Year
1976
Tongue
French
Weight
284 KB
Volume
12
Category
Article
ISSN
0022-2135

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✦ Synopsis

Abstract

^3^H‐Ochratoxins A, B, and C were obtained by tritiation of the toxins with tritiated water in acetic acid and platinum at 80Β° C overnight. The toxins were purified by solvent partition and thin layer chromatography with the recovery yield being 80% and the specific activity being 2.6 – 3.0 Ci/mmol. Hydrolysis of ^3^H‐ochratoxin A with carboxypeptidase A revealed that 80.9% of the tritium in ochratoxin A molecule distributed on the phenylalanine residue with only 19.1% in ochratoxin a moiety.

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