𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Preparation and isolation of immunologically active glycopeptides from carcinoembryonic antigen (CEA)

✍ Scribed by Dr. C. Banjo; Dr. P. Gold; C. W. Gehrke; S. O. Freedman; J. Krupey

Publisher
John Wiley and Sons
Year
1974
Tongue
French
Weight
779 KB
Volume
13
Category
Article
ISSN
0020-7136

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

In order to determine the structure of the tumor‐specific immunodominant group of the carcinoembryonic antigen (CEA) of human gastro‐intestinal tumors, immunologically active fragments from this glycoprotein were prepared by partial enzymatic hydrolysis using the proteolytic enzyme nagase. The fragments were purified by sequential molecular sieve, adsorption and ion exchange chromatography, and were analysed for carbohydrate and amino‐acid composition by gas‐liquid chromatography. The antigenic activity of each fragment was tested using both the Z‐gel and Farr radioimmunoassay techniques for CEA. Immunologically active glycopeptides with molecular weights of 1,000–5,000 Daltons, which contained the tumor antigenic determinant of CEA, had relatively high contents of N‐acetyl‐D‐glucosamine, aspartic acid or asparagine, and glutamic acid or glutamine.

📜 SIMILAR VOLUMES

Isolation and characterization of a homo
Isolation and characterization of a homogeneous isomeric species of carcinoembryonic antigen: CEA-S
✍ Edward F. Plow; Thomas S. Edgington 📂 Article 📅 1975 🏛 John Wiley and Sons 🌐 French ⚖ 910 KB

## Abstract A single homogeneous isomeric species of carcinoembryonic antigen was isolated by reference to solubility in 0.9 M perchloric acid, isoelectric focusing, molecular exclusion chromatography, ion exchange chromatography, passage through immunoabsorbants, and isopyknic density gradient ult

Isolation from human gastric juice of an
Isolation from human gastric juice of an antigen closely related to the carcinoembryonic antigen
✍ Matti Vuento; Eva Engvall; Markku Seppälä; Erkki Ruoslahti 📂 Article 📅 1976 🏛 John Wiley and Sons 🌐 French ⚖ 489 KB

## Abstract A carcinoembryonic antigen‐like antigen(s) (CELIA) was isolated from normal human gastric juice by immunoadsorbents and gel chromatography. The isolated CELIA reacted identically to CEA in immunodiffusion tests against anti‐CEA and anti‐CELIA. Other experiments suggested subtle differen