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Isolation and characterization of a homogeneous isomeric species of carcinoembryonic antigen: CEA-S

✍ Scribed by Edward F. Plow; Thomas S. Edgington

Publisher
John Wiley and Sons
Year
1975
Tongue
French
Weight
910 KB
Volume
15
Category
Article
ISSN
0020-7136

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✦ Synopsis

Abstract

A single homogeneous isomeric species of carcinoembryonic antigen was isolated by reference to solubility in 0.9 M perchloric acid, isoelectric focusing, molecular exclusion chromatography, ion exchange chromatography, passage through immunoabsorbants, and isopyknic density gradient ultracentrifugation. The final product, representing approximately 1.8% of the perchloric acid soluble glycoprotein of the tumor, is homogeneous and devoid of other proteins by polyacrylamide gel electrophoresis. This single species of carcinoembryonic antigen, CEA‐S, has a sedimentation velocity of 6.6, a diffusion constant of 3.05 × 10^−7^ cm^2^/sec, a mean Stokes radius of 65 Å, a density of 1.41 ml/g in cesium chloride and an estimated molecular weight of 181,000, and it is devoid of detectable A or B blood‐group antigens. Immunochemical studies demonstrate qualitative similarities between CEA‐S and conventional carcinoembryonic antigens; however, competitive inhibition analyses demonstrate significant quantitative immunochemical differences between CEA‐S and preparations of carcinoembryonic antigen. These results are consistent with the concept that CEA‐S is an immunochemical isomer of carcinoembryonic antigen.

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