𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Preparation and characterization of monoclonal antibodies specific to synthetic peptide of carcinoembryonic antigen

✍ Scribed by Masayuki Tsujisaki; Kohzoh Imai; Noriyuki Hishikawa; Shigeru Tokuchi; Yuji Hinoda; Hirokazu Matsukawa; Shinzo Oikawa; Hiroshi Nakazato; Akira Yachi

Publisher
John Wiley and Sons
Year
1991
Tongue
French
Weight
847 KB
Volume
47
Category
Article
ISSN
0020-7136

No coin nor oath required. For personal study only.

✦ Synopsis

Monoclonal antibodies (MAbs) were prepared to a synthetic peptide (PI : I I94 40 amino acids) in domain I of the carcinoembryonic antigen (CEA) molecule. The majority of the amino acids in peptide P I show a hydrophilic character, and similar sequences are repeated in domains I, II and 111 of this molecule. These MAbs appear to recognize new epitopes of CEA, since representative conventional MAbs to CEA do not show reactivity to synthetic peptide PI. The resulting MAbs were divided into 2 groups; group I (MAb PI-706) reacted

with peptide PI, but not with purified CEA preparation, while group 2 (MAb P 1-234 and P 1-255) reacted with either type of antigen, in the immunoblotting assay using purified CEA and in immunostaining of colonic carcinoma tissues. Furthermore, group-l MAb P 1-706 was reactive to purified CEA after treatment with periodate.

πŸ“œ SIMILAR VOLUMES

Generation, immunologic characterization
Generation, immunologic characterization and antitumor effects of human monoclonal antibodies for carcinoembryonic antigen
✍ Takayuki Imakiire; Motomu Kuroki; Hirotomo Shibaguchi; Hironori Abe; Yasushi Yam πŸ“‚ Article πŸ“… 2003 πŸ› John Wiley and Sons 🌐 French βš– 125 KB

## Abstract We generated fully human mAbs (HmAbs) to carcinoembryonic antigen (CEA) using the KM mouse, which carries a human chromosome 14 fragment containing the entire Ig H chain loci and human ΞΊ L chain segments in the mouse genome. Forty‐six hybridoma clones producing HmAbs to CEA were thus ob

Preparation and characterization of anti
Preparation and characterization of anti-anti-idiotypic monoclonal antibody (β€œAb1-like Ab3”) in relation to carcinoembryonic antigen
✍ Masayuki Tsujisaki; Jiro Masuya; Yoshimasa Okada; Tsuneharu Jinnohara; Shigeru S πŸ“‚ Article πŸ“… 2002 πŸ› John Wiley and Sons 🌐 English βš– 332 KB

## Abstract In order to analyze the epitope structure of carcinoembryonic antigen (CEA) and the idiotype network system, seven anti‐idiotypic monoclonal antibodies (anti‐Id MoAbs) were generated from a BALB/c mouse immunized with anti‐CEA MoAb P1‐356, which recognized a synthetic peptide P1 of CEA,

Characterization of a monoclonal antibod
Characterization of a monoclonal antibody specific to rainbow trout thrombocytes
✍ Kfoury, Jose Roberto; Nakayasu, Chihaya; Rodrigues Souza, Juan Carlos; Okamoto, πŸ“‚ Article πŸ“… 1999 πŸ› John Wiley and Sons 🌐 English βš– 614 KB

A monoclonal antibody (MAb) specific for rainbow trout thrombocytes was produced and its reactivity was demonstrated by flow cytometry and immuno-electron microscopy. Flow cytometry analysis showed that this MAb (TTL-7D11) reacted positively with about 30% of the peripheral blood leucocytes (PBL) an