## Abstract We generated fully human mAbs (HmAbs) to carcinoembryonic antigen (CEA) using the KM mouse, which carries a human chromosome 14 fragment containing the entire Ig H chain loci and human ΞΊ L chain segments in the mouse genome. Fortyβsix hybridoma clones producing HmAbs to CEA were thus ob
Characterization of second-generation monoclonal antibodies against carcinoembryonic antigen
β Scribed by Hans J. Hansen; Edward S. Newman; Ruth Grebenau; David M. Goldenberg; Robert M. Sharkey
- Publisher
- John Wiley and Sons
- Year
- 1993
- Tongue
- English
- Weight
- 816 KB
- Volume
- 71
- Category
- Article
- ISSN
- 0008-543X
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
B14-2-14 is a monoclonal cytotoxic IgM antibody which reacts with thymocytes of all mouse strains tested. The fraction of positive cells (by visual immunofluorescence) varies between strains from about 25-45% in A.CA to 65-85% in C57BL/6, and high levels are dominant in F1 hybrids. In the periphery,
Anti-actin monoclonal antibodies were prepared using phalloidin-stabilized actin that was purified from pea roots by DNase I affinity chromatography. One monoclonal antibody, designated mAb3H 1 1, bound plant actin in preliminary screenings and was further analyzed. Immunoblot analysis showed that t