Prediction of retention times of hydroxylated triacylglycerols in reversed-phase high-performance liquid chromatography
β Scribed by Lutz Haalck; Friedrich Spener
- Publisher
- Elsevier Science
- Year
- 1990
- Tongue
- English
- Weight
- 300 KB
- Volume
- 498
- Category
- Article
- ISSN
- 1873-3778
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π SIMILAR VOLUMES
Triacylglycerols have been separated by normal-phase high-performance liquid chromatography (HPLC) on silica utilising a solvent system consisting of dry acetonitrile-half water saturated hexane (0.7:99.3). This solvent system is UV transparent allowing detection at 200 nm and affords a separation i
## Abstract The ability of reversedβphase highβperformance liquid chromatography (RPβHPLC) to separate some positionally isomeric disaturated and monounsaturated triacylglycerols (TAGs) as intact species is demonstrated for the first time. Mobile phases of acetonitrile modified with methanol, ethan
Methanol-water compositions were used to empirically predict the retention of unknown hydroxyl aromatic compounds at different mobile phase compositions on a PBondapak Cl8 column. A single experimental k' value was needed to predict k' values at other mobile phase compositions for an unknown compoun