Prediction of retention of hydroxyl aromatics in reversed-phase liquid chromatography with slope-intercept relationships

✍ Scribed by Howard A. Cooper; Robert J. Hurtubise

• Publisher: Elsevier Science
• Year: 1986
• Tongue: English
• Weight: 773 KB
• Volume: 360
• Category: Article
• ISSN: 1873-3778
• DOI: 10.1016/s0021-9673(00)91682-0

✦ Synopsis

Methanol-water compositions were used to empirically predict the retention of unknown hydroxyl aromatic compounds at different mobile phase compositions on a PBondapak Cl8 column. A single experimental k' value was needed to predict k' values at other mobile phase compositions for an unknown compound. The method of predicting retention was based on two linear relationships derived from the experimental data. A linear relationship was obtained from plots of log k' as a function of the volume fraction of methanol in the mobile phase for standard hydroxyl compounds. Then a linear relationship was obtained by plotting the slope as a function of the intercept from the slope and intercept values derived from the log k' plots. Together, the two linear relationships permitted the prediction of retention 'at different mobile phase compositions for compounds structurally similar to the original data set.