𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Normal-phase high-performance liquid chromatography of triacylglycerols

✍ Scribed by S.H. Rhodes; A.G. Netting

Publisher
Elsevier Science
Year
1988
Tongue
English
Weight
534 KB
Volume
448
Category
Article
ISSN
1873-3778

No coin nor oath required. For personal study only.

✦ Synopsis

Triacylglycerols have been separated by normal-phase high-performance liquid chromatography (HPLC) on silica utilising a solvent system consisting of dry acetonitrile-half water saturated hexane (0.7:99.3). This solvent system is UV transparent allowing detection at 200 nm and affords a separation in which retention is primarily dependent on the number of constituent double bonds. There is also a slight separation on chainlength, the longer chainlengths being eluted first. The system is therefore complementary to currently used reversed-phase HPLC systems. Chromatograms for some polyunsaturated fats and oils are given, and the most polyunsaturated triacylglycerols from linseed oil are analysed in more detail. Data are given for the separation and quantitation of the pentafluorobenzyl esters of constituent fatty acids from these triacylglycerols by a similar normal-phase HPLC system.

πŸ“œ SIMILAR VOLUMES

Practical High-Performance Liquid Chroma
Practical High-Performance Liquid Chromatography (Meyer/Practical High-Performance Liquid Chromatography) || Adsorption Chromatography: Normal-Phase Chromatography
✍ Meyer, Veronika R. πŸ“‚ Article πŸ“… 2010 πŸ› John Wiley & Sons, Ltd 🌐 English βš– 706 KB

Jump into the HPLC adventure! Three decades on from publication of the 1st German edition of Veronika Meyer's book on HPLC, this classic text remains one of the few titles available on general HPLC aimed at practitioners. New sections on the following topics have been included in this fifth editio

Resolution of triacylglycerol positional
Resolution of triacylglycerol positional isomers by reversed-phase high-performance liquid chromatography
✍ Svetlana Momchilova; Koichiro Tsuji; Yutaka Itabashi; Boryana Nikolova-Damyanova πŸ“‚ Article πŸ“… 2004 πŸ› John Wiley and Sons 🌐 English βš– 254 KB

## Abstract The ability of reversed‐phase high‐performance liquid chromatography (RP‐HPLC) to separate some positionally isomeric disaturated and monounsaturated triacylglycerols (TAGs) as intact species is demonstrated for the first time. Mobile phases of acetonitrile modified with methanol, ethan

Separation of gibberellins by normal-pha
Separation of gibberellins by normal-phase high-performance liquid chromatography
✍ Per Christer OdΓ©n; Ludger Schwenen; Jan E. Graebe πŸ“‚ Article πŸ“… 1991 πŸ› Elsevier Science 🌐 English βš– 436 KB

A major difficulty with the study of endogenous gibberellins (GAS) in plants is to remove substances interfering with the subsequent qualitative or quantitative analyses, for a general review see, e.g., ref. 1. Since vegetative plant tissues usually contain small amounts of GAS together with large a

Regioselective separation of isomeric tr
Regioselective separation of isomeric triacylglycerols by reversed-phase high-performance liquid chromatography: Stationary phase and mobile phase effects
✍ Svetlana Momchilova; Yutaka Itabashi; Boryana Nikolova-Damyanova; Arnis Kuksis πŸ“‚ Article πŸ“… 2006 πŸ› John Wiley and Sons 🌐 English βš– 877 KB

## Abstract Complete regioselective separation of five pairs of isomeric dipalmitoyl polyalkenoyl glycerols with two to six double bonds in the unsaturated acyl residues has been achieved by RP‐HPLC on a single ODS column. Four ODS columns with stationary phases containing different percentages of