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Precursor-Directed Biosynthesis of 6-Deoxyerythronolide B Analogs in Streptomycescoelicolor: Understanding Precursor Effects

✍ Scribed by Timothy Leaf; Lawrence Cadapan; Christopher Carreras; Rika Regentin; Sally Ou; Elaine Woo; Gary Ashley; Peter Licari

Book ID
109386128
Publisher
American Institute of Chemical Engineers
Year
2000
Tongue
English
Weight
66 KB
Volume
16
Category
Article
ISSN
8756-7938

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📜 SIMILAR VOLUMES

Precursor directed biosynthesis of novel
Precursor directed biosynthesis of novel 6-deoxyerythronolide B analogs containing non-natural oxygen substituents and reactive functionalities
✍ Daniel Hunziker; Nicholas Wu; Kenji Kenoshita; David E. Cane; Chaitan Khosla 📂 Article 📅 1999 🏛 Elsevier Science 🌐 French ⚖ 258 KB

Feeding of synthetic precursors to a blocked mutant of 6-deoxyerythronoltde B synthase (DEBS) [1] led to production of novel 6-deoxyerythronolide B analogs in vivo containing additional non-natural oxygen substituents as well as additional reactive groups.

Employing racemic precursors in directed
Employing racemic precursors in directed biosynthesis of 6-dEB analogs
✍ Timothy Leaf; Mark Burlingame; Ruchir Desai; Rika Regentin; Elaine Woo; Gary Ash 📂 Article 📅 2002 🏛 Wiley (John Wiley & Sons) 🌐 English ⚖ 133 KB

## Abstract Substitution of racemic diketide thioesters for optically pure compounds in precursor‐fed fermentations was investigated. These substrates were shown to be as effective as optically pure materials in diketide‐fed fermentation processes for producing analogs of 6‐deoxyerythronolide B. Ho

Precursor-Directed Biosynthesis: Stereos
Precursor-Directed Biosynthesis: Stereospecificity for Branched-Chain Diketides of the β-Ketoacyl-ACP Synthase Domain 2 of 6-Deoxyerythronolide B Synthase
✍ Kenji Kinoshita; Chaitan Khosla; David E. Cane 📂 Article 📅 2003 🏛 John Wiley and Sons 🌐 German ⚖ 330 KB

## Abstract Modular polyketide synthases such as 6‐deoxyerythronolide B synthase (DEBS) catalyze the biosynthesis of structurally complex natural products. __Streptomyces coelicolor__ CH999/pJRJ2 harbors a plasmid encoding DEBS(KS1^0^), a mutant form of 6‐deoxyerythronolide B synthase that is block