Precursor-directed biosynthesis of 6-deoxyerythronolide B analogues is improved by removal of the initial catalytic sites of the polyketide synthase

Precursor-Directed Biosynthesis: Stereos

Precursor-Directed Biosynthesis: Stereospecificity for Branched-Chain Diketides of the β-Ketoacyl-ACP Synthase Domain 2 of 6-Deoxyerythronolide B Synthase

✍ Kenji Kinoshita; Chaitan Khosla; David E. Cane 📂 Article 📅 2003 🏛 John Wiley and Sons 🌐 German ⚖ 330 KB

## Abstract Modular polyketide synthases such as 6‐deoxyerythronolide B synthase (DEBS) catalyze the biosynthesis of structurally complex natural products. __Streptomyces coelicolor__ CH999/pJRJ2 harbors a plasmid encoding DEBS(KS1^0^), a mutant form of 6‐deoxyerythronolide B synthase that is block