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Post-source decay analysis in matrix-assisted laser desorption/ionization mass spectrometry of biomolecules

โœ Scribed by Spengler, Bernhard

Publisher
John Wiley and Sons
Year
1997
Tongue
English
Weight
659 KB
Volume
32
Category
Article
ISSN
1076-5174

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โœฆ Synopsis

An introduction to primary structure analysis of biomolecules by matrix-assisted laser desorption/ionization (MALDI) post-source decay (PSD) is given, sketching the principles and applications of the method for scientists in molecular biology, biochemistry and biomedicine. The fundamentals of PSD are described in order to explain the potential and limitations of its applicability. Two examples of peptide sequencing of a completely unknown peptide and of a database-listed peptide are presented and the procedure of (non-automated) amino acid sequence elucidation is described.

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