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Protein sequence information by matrix-assisted laser desorption/ionization in-source decay mass spectrometry

✍ Scribed by Julie Hardouin

Publisher
John Wiley and Sons
Year
2007
Tongue
English
Weight
176 KB
Volume
26
Category
Article
ISSN
0277-7037

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✦ Synopsis

Abstract

Proteins from biological samples are often identified by mass spectrometry (MS) with the two following β€œbottom‐up” approaches: peptide mass fingerprinting or peptide sequence tag. Nevertheless, these strategies are time‐consuming (digestion, liquid chromatography step, desalting step), the N‐ (or C‐) terminal information often lacks and post‐translational modifications (PTMs) are hardly observed. The in‐source decay (ISD) occurring in a matrix assisted laser desorption/ionization (MALDI) source appears an interesting analytical tool to obtain N‐terminal sequence, to identify proteins and to characterize PTMs by a β€œtop‐down” strategy. The goal of this review deals with the usefulness of the ISD technique in MALDI source in proteomics fields. In the first part, the ISD principle is explained and in the second part, the use of ISD in proteomic studies is discussed for protein identification and sequence characterization. Β© 2007 Wiley Periodicals, Inc., Mass Spec Rev 26:672–682, 2007

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