Positively charged macromolecules prevent fertilization of sea urchin eggs
β Scribed by G. Czihak; I. Czihak
- Book ID
- 105048315
- Publisher
- Springer
- Year
- 1981
- Tongue
- English
- Weight
- 217 KB
- Volume
- 68
- Category
- Article
- ISSN
- 0028-1042
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release was induced by intracoelomically treating the specimens with 0.5M KC1. The harvested eggs were then rinsed thoroughly in Instant Ocean, (Aquarium Systems Co., Mentor, Ohio) (pH 7.801, a synthetic marine water mix. Male urchin testes were removed, placed in a petri dish, and maintained at 4Β°
## Abstract βFertilization acidβ is released from sea urchin eggs upon fertilization and decreases the pH of the surrounding seawater. In bicarbonateβfree artificial seawater flushed with nitrogen gas, the pH shift still occurs but returns to the original value in a few minutes, suggesting that the