Rhodamine (rh) phalloidin was used as a probe for actin filaments in living eggs and was observed using confocal microscopy. Exogenous, rh phalloidin labeled actin filaments were injected into eggs and were observed to translocate in the cytoplasm. At fertilization, filaments accumulated at the fert
Redistribution of actin and fascin in sea urchin eggs after fertilization
โ Scribed by Otto, J. J. ;Kane, R. E. ;Bryan, J.
- Publisher
- Wiley (John Wiley & Sons)
- Year
- 1980
- Weight
- 679 KB
- Volume
- 1
- Category
- Article
- ISSN
- 0271-6585
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
## Abstract The pH versus proteinase activity curve (casein or hemoglobin plus urea substrate) for homogenates of unfertilized __Lytechinus__ eggs reveals two regions of maximum activity: one between pH 3.5 and 4.3, and another of far greater magnitude from pH 8.0 to 11.0. The two classes of protei
release was induced by intracoelomically treating the specimens with 0.5M KC1. The harvested eggs were then rinsed thoroughly in Instant Ocean, (Aquarium Systems Co., Mentor, Ohio) (pH 7.801, a synthetic marine water mix. Male urchin testes were removed, placed in a petri dish, and maintained at 4ยฐ
## Abstract Ghosts were prepared from sea urchin eggs, before and after fertilization. Eggs were treated with Mg^++^ to stabilize the surface. Cytoplasm was then released by osmotically lysing the cells and washing repeatedly at low ionic strength. Microscopically, the resultant ghosts appear to co
Elongation of microvilli and formation of actin filaments after treatment with a phorbol ester, TPA, were investigated in unfertilized eggs of Hemicentrotus pulcherrimus. Microvilli on the egg surface were examined by scanning electron microscopy. Actin filaments in the cortical layer of the eggs we