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Characterization of water in unfertilized and fertilized sea urchin eggs

✍ Scribed by Philip J. Merta; Gary D. Fullerton; Ivan L. Cameron

Publisher: John Wiley and Sons
Year: 1986
Tongue: English
Weight: 920 KB
Volume: 127
Category: Article
ISSN: 0021-9541
DOI: 10.1002/jcp.1041270313

No coin nor oath required. For personal study only.

✦ Synopsis

release was induced by intracoelomically treating the specimens with 0.5M KC1. The harvested eggs were then rinsed thoroughly in Instant Ocean, (Aquarium Systems Co., Mentor, Ohio) (pH 7.801, a synthetic marine water mix.

Male urchin testes were removed, placed in a petri dish, and maintained at 4°C until use. Sperm solutions consisted of one drop semen diluted with 2 ml sea water;

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