On the timing and activation of glycogen catabolism in fertilized sea urchin eggs
β Scribed by Baginski, Richard M.
- Publisher
- John Wiley and Sons
- Year
- 1981
- Tongue
- English
- Weight
- 259 KB
- Volume
- 216
- Category
- Article
- ISSN
- 0022-104X
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β¦ Synopsis
Abstract
Decreased glycogen radioactivity was followed as an indicator of glycogen utilization following activation of Strongylocentrotus purpuratus eggs. Within 15 minutes of exposure to sperm or ammonia, egg glycogen radioactivity decreased. The level of radioactivity in control (unfertilized) eggs remained relatively constant. These results suggest enhanced egg glycogenolysis is a pHβsensitive event independent of the βrespiratory burstβ.
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When sea urchin eggs are pretreated with fluorescent chelate probe chlorotetracycline (CTC) and then fertilized with unlabeled sperm, a small, brightly fluorescent particle resembling the mitochondrion of free-swimming sperm both in size and fluorescent staining characteristics appears in the egg cy