## Abstract Decreased glycogen radioactivity was followed as an indicator of glycogen utilization following activation of __Strongylocentrotus purpuratus__ eggs. Within 15 minutes of exposure to sperm or ammonia, egg glycogen radioactivity decreased. The level of radioactivity in control (unfertili
Uptake OF Ca2+ is one of the earliest responses to fertilization of sea urchin eggs
β Scribed by Paul, Miles ;Johnston, Randal N.
- Publisher
- John Wiley and Sons
- Year
- 1978
- Tongue
- English
- Weight
- 351 KB
- Volume
- 203
- Category
- Article
- ISSN
- 0022-104X
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β¦ Synopsis
Abstract
Sea urchin eggs take up Ca for 10 min following insemination (determined as uptake of ^45^Ca from the SW (3)). Although 90% of this uptake occurs after the beginning of the cortical reaction and may represent external binding of Ca to the egg surface coats, there is a brief phase of uptake (0β30 sec) which precedes the cortical reaction; this may represent a Ca flux into the eggs.
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Changes in the intracellular Ca2+ concentration ([Ca2+]i) and the formation of actin filaments were investigated in unfertilized eggs of the sea urchin Hemicentrotus pulcherrimus after activation with a phorbol ester, 12-O-tetradecanoyl phorbol13-acetate (TPA). Intracellular Ca2+ oscillation was obs
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