✦ LIBER ✦

Polyspermy-preventing mechanisms in mouse eggs fertilized in vitro

✍ Scribed by Sato, Kodo

Publisher: John Wiley and Sons
Year: 1979
Tongue: English
Weight: 283 KB
Volume: 210
Category: Article
ISSN: 0022-104X
DOI: 10.1002/jez.1402100219

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

The time sequence of the mechanisms that prevent polyspermy in cumulus‐free mouse eggs was determined by continuous microscopic observations during in vitro fertilization. The zona reaction had begun, and the vitelline surface block to polyspermy was established, in less than one minute following sperm fusion with the vitellus. In less than five minutes, the zona reaction had advanced to the stage that no more spermatozoa could become firmly attached to the surface of the zona pellucida.

📜 SIMILAR VOLUMES

The male factor in fertilization of rat

The male factor in fertilization of rat eggs in vitro

✍ Shalgi, Ruth ;Kaplan, Ruth ;Nebel, L. ;Kraicer, P. F. 📂 Article 📅 1981 🏛 John Wiley and Sons 🌐 English ⚖ 321 KB 👁 1 views

## Abstract Both epididymal and ejaculated spermatozoa give usable rates of fertilization of superovulated oocytes in vitro. The epididymal sperm gave higher rates, but ejaculated sperm were preferred because intra‐donor variation was lower than inter‐donor variation. A selected population of 14 ma

In vitro fertilization and polyspermy in

In vitro fertilization and polyspermy in the pig: Factors affecting fertilization rates and cytoskeletal reorganization of the oocyte

✍ Hiroyuki Suzuki; Yosuke Saito; Noriko Kagawa; Xiangzhong Yang 📂 Article 📅 2003 🏛 John Wiley and Sons 🌐 English ⚖ 486 KB

## Abstract Polyspermy is a common phenomenon in the pig. Extensive information has become available from in vitro studies on not only the quality of oocytes but also the quality of spermatozoa. However, little information is available on the relative penetration rates of fresh and frozen spermatoz

Cellular and molecular mechanisms leadin

Cellular and molecular mechanisms leading to cortical reaction and polyspermy block in mammalian eggs

✍ Qing-Yuan Sun 📂 Article 📅 2003 🏛 John Wiley and Sons 🌐 English ⚖ 150 KB

## Abstract Following fusion of sperm and egg, the contents of cortical granules (CG), a kind of special organelle in the egg, release into the perivitelline space (cortical reaction), causing the zona pellucida to become refractory to sperm binding and penetration (zona reaction). Accumulating evi

In vivo and in vitro fertilization of ha

In vivo and in vitro fertilization of hamster, rat and mouse eggs after treatment with anti-hamster ovary antiserum

✍ Tsunoda, Y. ;Chang, M. C. 📂 Article 📅 1976 🏛 John Wiley and Sons 🌐 English ⚖ 446 KB 👁 1 views

## Abstract Rabbit antiserum against hamster ovary was examined on agargel diffusion plates against several hamster tissues, and also against rat and mouse ovarian extracts. Unabsorbed anti‐hamster ovary antiserum showed eight to nine precipitin bands for hamster ovary and four to eight bands for o

Effect of polyamines on fertilization of

Effect of polyamines on fertilization of mouse ova in vitro

✍ Stanger, J. D. ;Quinn, P. 📂 Article 📅 1982 🏛 John Wiley and Sons 🌐 English ⚖ 343 KB 👁 1 views

## Abstract After a 1‐hour preincubation of epididymal mouse spermatozoa at a concentration of 2 × 10^6^/ml in 0.23 mM spermine, the proportion of F~1~ (C57BL × CBA) mouse ova fertilized after 1 and 2 hours was significantly greater than with untreated spermatozoa. Spermine also significantly incre

Effects of PKC ζ on early genome transcr

Effects of PKC ζ on early genome transcription activation in mouse 1-cell stage fertilized eggs

✍ Bing-zhi Yu; Wei Fu; Wen-hui Su; Da-hai Yu; Zhe Zhang; Chen Feng 📂 Article 📅 2007 🏛 John Wiley and Sons 🌐 English ⚖ 119 KB

Effects of PKC z on the activation of embryonic transcription in 1-cell stage fertilized mouse eggs were explored. The effects of PKC antagonist calphostin C and PKC z specific inhibitor on the activation of embryonic early transcription were observed by Western blotting and cell immunofluorescence.