Effects of PKC ζ on early genome transcription activation in mouse 1-cell stage fertilized eggs
✍ Scribed by Bing-zhi Yu; Wei Fu; Wen-hui Su; Da-hai Yu; Zhe Zhang; Chen Feng
- Publisher
- John Wiley and Sons
- Year
- 2007
- Tongue
- English
- Weight
- 119 KB
- Volume
- 25
- Category
- Article
- ISSN
- 0263-6484
- DOI
- 10.1002/cbf.1357
No coin nor oath required. For personal study only.
✦ Synopsis
Effects of PKC z on the activation of embryonic transcription in 1-cell stage fertilized mouse eggs were explored. The effects of PKC antagonist calphostin C and PKC z specific inhibitor on the activation of embryonic early transcription were observed by Western blotting and cell immunofluorescence. PKC activity increased gradually from G 1 phase to late G 2 phase in mouse 1-cell stage fertilized eggs, and reached a maximum in G 2 stage. Calphostin C inhibited PKC activity by about 47% in 1-cell stage fertilized eggs. Calphostin C inhibited early transcription in 1-cell stage fertilized eggs (p < 0.01). PKC z-Thr410 in G 2 were about 27% and 110% higher than those in G 1 phase of 1-cell stage fertilized eggs and MII oocytes, respectively. PKC z specific inhibitor can also inhibit early transcription in 1-cell stage fertilized eggs (p < 0.05). The results suggest that PKC z participates in early transcription activation in mouse 1-cell stage fertilized eggs.