𝔖 Bobbio Scriptorium
✦   LIBER   ✦

The male factor in fertilization of rat eggs in vitro

✍ Scribed by Shalgi, Ruth ;Kaplan, Ruth ;Nebel, L. ;Kraicer, P. F.

Publisher
John Wiley and Sons
Year
1981
Tongue
English
Weight
321 KB
Volume
217
Category
Article
ISSN
0022-104X

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Both epididymal and ejaculated spermatozoa give usable rates of fertilization of superovulated oocytes in vitro. The epididymal sperm gave higher rates, but ejaculated sperm were preferred because intra‐donor variation was lower than inter‐donor variation. A selected population of 14 male donors had normally distributed fertilizing capacity with a mean and standard deviation of 77 Β± 14% (2,823 eggs). Successive ejaculates usually gave similar rates of fertilization. An attempt was made to eliminate dead and agglutinated cells from the ejaculated sperm by filtration through a column of glass beads. Despite the expected marked improvement in the average motility, the eluted spermatozoa were incapable of fertilizing. We concluded that the preferred source of spermatozoa for fertilization in vitro is ejaculated sperm. In the rat, motility and cell morphology are not adequate criteria for fertilizing capacity of treated spermatozoa.

πŸ“œ SIMILAR VOLUMES

The effect of sperm concentrations durin
The effect of sperm concentrations during preincubation and at insemination on fertilization of rat eggs in vitro
✍ Nishimura, H. ;Niwa, K. ;Miyake, M. ;Iritani, A. πŸ“‚ Article πŸ“… 1982 πŸ› John Wiley and Sons 🌐 English βš– 298 KB πŸ‘ 2 views

## Abstract Rat epididymal spermatozoa, at a concentration of 40–65 Γ— 10^4^ cells/ml, were preincubated for 5–5.5 hr and then added to eggs from superovu‐lated, immature rats. By 10–12 hr after insemination, 86% of the eggs had undergone fertilization, but many of them (41%) were polyspermic. When

In vivo and in vitro fertilization of ha
In vivo and in vitro fertilization of hamster, rat and mouse eggs after treatment with anti-hamster ovary antiserum
✍ Tsunoda, Y. ;Chang, M. C. πŸ“‚ Article πŸ“… 1976 πŸ› John Wiley and Sons 🌐 English βš– 446 KB πŸ‘ 1 views

## Abstract Rabbit antiserum against hamster ovary was examined on agargel diffusion plates against several hamster tissues, and also against rat and mouse ovarian extracts. Unabsorbed anti‐hamster ovary antiserum showed eight to nine precipitin bands for hamster ovary and four to eight bands for o

Influence of hernioplastic implants on m
Influence of hernioplastic implants on male fertility in rats
✍ T. Kolbe; W. Lechner πŸ“‚ Article πŸ“… 2007 πŸ› John Wiley and Sons 🌐 English βš– 364 KB πŸ‘ 1 views

## Abstract This study explored the vulnerability of the ductus deferens due to mesh induced inflammation and shrinkage after hernia repair in the rodent model. Two commonly used types of hernioplastic implants (Proleneβ„’ and Vypro IIβ„’) were surgically wrapped around the ductus deferentes on both si

In vitro fertilization and polyspermy in
In vitro fertilization and polyspermy in the pig: Factors affecting fertilization rates and cytoskeletal reorganization of the oocyte
✍ Hiroyuki Suzuki; Yosuke Saito; Noriko Kagawa; Xiangzhong Yang πŸ“‚ Article πŸ“… 2003 πŸ› John Wiley and Sons 🌐 English βš– 486 KB

## Abstract Polyspermy is a common phenomenon in the pig. Extensive information has become available from in vitro studies on not only the quality of oocytes but also the quality of spermatozoa. However, little information is available on the relative penetration rates of fresh and frozen spermatoz

Effect of elevated calcium concentration
Effect of elevated calcium concentration on fertilization of rat oocytes in vitro
✍ Kaplan, Ruth ;Kraicer, P. F. πŸ“‚ Article πŸ“… 1978 πŸ› Wiley (John Wiley & Sons) βš– 317 KB πŸ‘ 1 views

The purpose of this study was t o develop a modification of the medium of Toyoda and Chang which would permit high rates of fertilization in vitro in rats of our colony. Elevation of the calcium concentration from 1.7 1 t o 3.42 mM increased the fertilization rate by 87%. Greater additions of calciu