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Effect of elevated calcium concentration on fertilization of rat oocytes in vitro

✍ Scribed by Kaplan, Ruth ;Kraicer, P. F.

Publisher: Wiley (John Wiley & Sons)
Year: 1978
Weight: 317 KB
Volume: 1
Category: Article
ISSN: 0148-7280
DOI: 10.1002/mrd.1120010308

No coin nor oath required. For personal study only.

✦ Synopsis

The purpose of this study was t o develop a modification of the medium of Toyoda and Chang which would permit high rates of fertilization in vitro in rats of our colony. Elevation of the calcium concentration from 1.7 1 t o 3.42 mM increased the fertilization rate by 87%. Greater additions of calcium gave no further improvement. This effect was not due t o reduction in phosphate activity, nor was it due t o antagonism of heavy-metal contamination. Although calcium is known t o enhance the fertilizing capacity of spermatozoa, our results suggest that it may also affect the oocyte.

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