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Inhibitory effect of dibutyryl cAMP on mouse oocyte maturation in vitro

✍ Scribed by Cho, Wan Kyoo ;Stern, Samuel ;Biggers, John D.

Publisher: John Wiley and Sons
Year: 1974
Tongue: English
Weight: 296 KB
Volume: 187
Category: Article
ISSN: 0022-104X
DOI: 10.1002/jez.1401870307

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

The effects of dibutyryl cyclic AMP on the maturation of the mouse oocyte in vitro were studied. It was found that concentrations of 100 μg/ml or greater prevented germinal vesicle breakdown in more than 95% of the treated oocytes. The inhibition of maturation was found to be a reversible phenomenon. When oocytes were exposed to the drug after the completion of germinal vesicle breakdown, no effect of the drug was noted. Thus, dibutyryl cyclic AMP appears to effect only the initial stages of meiotic maturation in the mouse oocyte.

Theophylline was also found to inhibit maturation, suggesting that increased levels of cyclic AMP in the oocyte may be the causative agent of the inhibition of maturation.

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