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Effect of dithiothreitol on meiotic maturation of mouse oocytes in vitro: Dependence of the effect on N6,O2′-dibutyryl adenosine 3′,5′-cyclic monophosphate

✍ Scribed by Wassarman, Paul M. ;Turner, Pamela E.

Publisher
John Wiley and Sons
Year
1976
Tongue
English
Weight
384 KB
Volume
196
Category
Article
ISSN
0022-104X

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✦ Synopsis

Abstract

The inhibitory effect of dithiothreitol on meiotic maturation of mouse oocytes in vitro is a function of the intracellular dibutyryl cyclic AMP concentration. Inhibition of nuclear (germinal vesicle) breakdown by dibutyryl cyclic AMP is reversed upon transfer of oocytes to plain culture medium, where‐as, transfer to medium containing dithiothreitol results in continued inhibition. Dithiothreitol significantly enhances the effectiveness of dibutyryl cyclic AMP as an inhibitor of meiotic maturation. These results suggest that the reported effect of sulfhydryl reducing agents on membrane dissolution and reconstitution, in both meiotic and mitotic cells, may be attributed to their influence on intracellular levels of cyclic AMP.

📜 SIMILAR VOLUMES

Meiotic maturation of the mammalian oocy
Meiotic maturation of the mammalian oocyte in vitro: Effect of dibutyryl cyclic AMP on protein synthesis
✍ Stern, Samuel ;Wassarman, Paul M. 📂 Article 📅 1974 🏛 John Wiley and Sons 🌐 English ⚖ 283 KB 👁 1 views

## Abstract Mouse oocytes arrested in dictyate of the first meiotic prophase by dibutyryl cyclic AMP accumulated exogenous valine at the same rate as oocytes undergoing meiotic maturation. The apparent rate of protein synthesis and the spectrum of proteins synthesized were not significantly affecte