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Polyoma virus replication in ascitic cells

✍ Scribed by Bo Nordenskjöld

Publisher
John Wiley and Sons
Year
1968
Tongue
French
Weight
352 KB
Volume
3
Category
Article
ISSN
0020-7136

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✦ Synopsis

Several ascitic tumors have been tested for polyoma sensitivity. Three tumors, one methylcholanthrene-induced and two polyoma-virus-induced, were demonstrated to be sensitive to polyoma virus as judged by plaque titration, hemagglutination and fluorescent antibody assay.

These determinants were correlated with the number of surviving cells and appearance of antiviral antibodies. The amount of virus produced and the number of infected cells found increased exponentially to reach a plateau about 50-100 hr post infection, after which the two respective counts either remained stationary or proceeded to fall.

A reduction in virus recovery was paralleled by the appearance of antiviral antibodies in the serum. The total number of cells present in theperitonealcavity remainedstationary, or dropped after polyoma infection, suggesting the possibility that polyoma virus acted as an oncolytic agent.

The high number of infected cells obtained at optimal times after polyoma exposure suggests the possibility that ascites tumors can be a useful routine tool for biochemical and other experiments where an abundant source of polyoma-infected cells is needed.

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