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Polymeric hollow fibers for capillary electrophoresis

✍ Scribed by Peter Z. Liu; Abdul Malik; Marvin C. J. Kuchar; William P. Vorkink; Milton L. Lee

Publisher
John Wiley and Sons
Year
1993
Tongue
English
Weight
620 KB
Volume
5
Category
Article
ISSN
1040-7685

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✦ Synopsis

Abstract

In this paper, polymeric hollow fibers prepared from polypropylene and polybutylene terephthalate were used as CE columns. Surface modifications with polyacrylamide greatly improved the performance of the capillaries. Separations of substituted pyridines, model proteins and standard ribonucleotides were demonstrated. The column efficiency for model proteins was over 200,000 theoretical plates. Comparisons of electroosmotic flow, UV absorption properties, heat dissipation, and diameter variation between fused silica capillaries and polymeric hollow fibers were made. The practical potential of small diameter polymeric hollow fibers as CE columns was evaluated.

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