𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Polyacrylamide-modified polypropylene hollow fibers for capillary electrophoresis

✍ Scribed by Peter Z. Liu; Abdul Malik; Marvin C. J. Kuchar; Milton L. Lee

Publisher
John Wiley and Sons
Year
1994
Tongue
English
Weight
648 KB
Volume
6
Category
Article
ISSN
1040-7685

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Surface treatment methods were developed for bonding of a polyacrylamide layer on the inside surface of polypropylene hollow fiber columns for capillary electrophoresis. Details of different modification procedures involving surface activation and in situ polymerization, using dynamic and static coating of reagents, are described. Both linear and cross‐linked polyacrylamide modified surfaces greatly improved the electrophoretic performance of the capillaries. The column efficiencies for model proteins reached as high as 370,000 theoretical plates on an 85 ΞΌm i.d. polyacrylamide surfacetreated polypropylene hollow fiber column. A variety of applications, including the separation of several very polar compounds at a pH of 10.6, were demonstrated. Reproducibility measurements were used to illustrate the performance and high pH stability of the resultant columns.

πŸ“œ SIMILAR VOLUMES

Cellulose modified polypropylene hollow
Cellulose modified polypropylene hollow fibers for capillary electrophoresis
✍ Xiaoli Ren; Peter Z. Liu; Milton L. Lee πŸ“‚ Article πŸ“… 1996 πŸ› John Wiley and Sons 🌐 English βš– 398 KB πŸ‘ 2 views

A new method for the modification of polypropylene hollow-fiber columns for capillary electrophoresis is reported. Chlorination was first used to activate the capillary inner surface. This was followed by treating the column with an alkaline solution containing polyhydroqpropylcellulose. A protein m

Polypropylene hollow fibers modified wit
Polypropylene hollow fibers modified with PMMA for capillary electrophoresis
✍ Shize Qi; Milton L. Lee πŸ“‚ Article πŸ“… 1998 πŸ› John Wiley and Sons 🌐 English βš– 117 KB πŸ‘ 2 views

A method for modification of polypropylene hollow fibers using graft Ε½ . copolymerization of polymethylmethacrylate PMMA was developed. The unsaturated residues in polypropylene were reacted with borane-dimethylsulfide, and PMMA was graft polymerized on the surface to form a stable coating which red

Hydrophilic polymer-modified polypropyle
Hydrophilic polymer-modified polypropylene hollow fibers with controllable electroosmotic flow for capillary electrophoresis
✍ Xiaoli Ren; Peter Z. Liu; Abdul Malik; Milton L. Lee πŸ“‚ Article πŸ“… 1996 πŸ› John Wiley and Sons 🌐 English βš– 470 KB πŸ‘ 2 views

Fused-silica capillaries often lack long-term stability and control of electroosmotic flow in capillary electrophoresis owing to the reactivity of the bulk silica structure in aqueous solutions of high or low pH. Polypropylene hollow fibers, an alternative to fused silica, have been chemically modif

Polymeric hollow fibers for capillary el
Polymeric hollow fibers for capillary electrophoresis
✍ Peter Z. Liu; Abdul Malik; Marvin C. J. Kuchar; William P. Vorkink; Milton L. Le πŸ“‚ Article πŸ“… 1993 πŸ› John Wiley and Sons 🌐 English βš– 620 KB

## Abstract In this paper, polymeric hollow fibers prepared from polypropylene and polybutylene terephthalate were used as CE columns. Surface modifications with polyacrylamide greatly improved the performance of the capillaries. Separations of substituted pyridines, model proteins and standard rib