Hydrophilic polymer-modified polypropylene hollow fibers with controllable electroosmotic flow for capillary electrophoresis

✍ Scribed by Xiaoli Ren; Peter Z. Liu; Abdul Malik; Milton L. Lee

• Publisher: John Wiley and Sons
• Year: 1996
• Tongue: English
• Weight: 470 KB
• Volume: 8
• Category: Article
• ISSN: 1040-7685
• DOI: 10.1002/(sici)1520-667x(1996)8:8<535::aid-mcs3>3.0.co;2-2

✦ Synopsis

Fused-silica capillaries often lack long-term stability and control of electroosmotic flow in capillary electrophoresis owing to the reactivity of the bulk silica structure in aqueous solutions of high or low pH. Polypropylene hollow fibers, an alternative to fused silica, have been chemically modified to control the electroosmotic flow. Ionic functional groups were used to vary or change the direction of electroosmotic flow. Polymeric surface coatings containing sulfonic acid and quaternary amine functionalities, and a novel polyphosphazene were bonded to the polypropylene surface and evaluated for electrophoresis and electroosmotic flow control. Varying magnitudes of electroosmotic flow were obtained with different concentrations or mixtures of these coatings. The resultant columns were successfully used for small-molecule separations, such as substituted pyridines and DNA bases. o 1997 John Wiley & Sons, tnc.